Studies on the hetrogeneity of lysine-rich histones in dividing cells.

Studies on histones. 8. A degradation product of lysine-rich histone.

Histones with High Lysine Content

1. The preparation and properties of lysine-rich histones, which differ in a number of respects from the classical arginine-rich histones, have been described. 2. Lysine-rich histones, like those previously known, are located in cell nuclei. 3. Lysine-rich histones dissociate more readily from combination with nucleic acid than do other histones.

The Relationship of the "sphere Chromatophile" to the Fate of Displaced Histones following Histone Transition in Rat Spermiogenesis

Cytochemical, radioautographic, and microspectrophotometric studies bearing on the relationship of histone transition to the origin and development of the protein and RNA components of the "sphère chromatophile" in the developing spermatogenic cells of the albino rat are presented. These studies show that the sphère chromatophile has many features in common with somatic nuclei: it contains hist...

Blocking by histones of accessibility to DNA in chromatin: addition of histones.

The blocking effect of histones on accessibility of DNA to DNase has been studied by addition of various histones to chromatin in intact or histone-depleted thymus nuclei (those from which all lysine-rich histone was removed and those from which much of the arginine-rich histone was removed) and to deoxyribonucleoprotein (chromatin) extracted from nuclei. In each case lysine-rich histone, weigh...

The role of lysine-rich histone in the maintenance of chromatin structure in metaphase chromosomes.

Dense masses of chromatin are present in the nuclei of many cells during interphase; dense chromatin is also present in the chromosomes of cells during mitosis. Relatively little RNA synthesis takes place in the dense chromatin of interphase cells,1' 2 and apparently none at all in the dense chromatin of metaphase chromosomes. It has been shown that chromatin threads of thymus nuclei are bound ...